酸枣叶黄酮的提取工艺优化 及其抗秀丽隐杆线虫氧化损伤活性

运用响应面法优化酸枣叶黄酮的超声辅助提取工艺,并探究其抗氧化活性。在单因素基础上,筛选出液料比、乙醇浓度、超声功率和超声时间并设计响应面实验。结果表明最优工艺条件为,液料比30.25:1 mL/g、乙醇浓度79.00%、超声功率301.00 W、超声时间32.00 min,酸枣叶黄酮实际提取率4.21%,与预测值4.51%相比,相对误差为6.65%,证明模型理论预测值与实际值拟合效果良好。制得的酸枣叶黄酮对DPPH自由基、ABTS自由基、羟自由基的半数清除浓度(IC₅₀)分别为111.51、23.06和253.73 μg/mL,具有较强的还原力。酸枣叶黄酮能有效增强秀丽隐杆线虫对热应激、H₂O₂氧化应激的抗性,抑制氧化损伤导致的线虫体内GSH-Px、SOD酶活力的降低,抑制MDA含量的增加,具有良好的抗氧化损伤作用。

Optimization of Extraction Process of Flavonoids from Ziziphus jujuba Mill var. spinosa Leaves and Its Antioxidant Damage Activity in Caenorhabditis elegans

To optimize the ultrasonic-assisted extraction process of flavonoids from Ziziphus jujuba Mill var. spinosa leaves(ZSL),the response surface methodology was used and its antioxidant activity was explored. Based on the single factor experiment,the ratio of liquid to material,ethanol concentration,ultrasonic power and ultrasonic time were selected and response surface experiment was designed. The optimal extraction conditions were as follows:Ratio of liquid to material 30.25:1 mL/g,ethanol concentration 79.00%,ultrasonic power 301.00 W,and ultrasonic time 32.00 min. The actual extraction rate of was 4.21%.The relative error was 6.65% compared with the predicted value of 4.51%,which proved that the theoretical predicted value of the model fitted well with the actual value. The half maximal inhibitory concentration(IC₅₀)of the flavonoids to DPPH,ABTS and ·OH radicals were 111.51,23.06 and 253.73 μg/mL,respectively,indicated that it had strong reducing power. The flavonoids from ZSL could effectively enhance the resistance to heat stress and H₂O₂ oxidative stress of Caenorhabditis elegans(C. elegans),inhibited the decrease of GSH-Px and SOD activity in worms caused by oxidative damage,inhibited the increasing of MDA content,and had a good role of the resistance to oxidative damage.