蒲公英黄酮类醇提物对食管鳞癌细胞增殖和侵袭的影响

目的:研究蒲公英黄酮类醇提物对人食管鳞癌细胞(Esophageal squamous cell Carcinoma,ESCC)增殖、迁移和侵袭的影响及作用机制。方法:采用CCK-8法检测蒲公英黄酮类醇提物对ESCC细胞增殖的影响;通过细胞划痕和Transwell迁移实验检测药物对ESCC细胞迁移和侵袭的影响;Western blot法初步分析药物对细胞迁移和侵袭的作用机制。结果:蒲公英黄酮类醇提物对食管鳞癌细胞增殖呈时间和浓度梯度依赖性,且随着浓度增加明显抑制ESCC细胞的迁移和侵袭;蒲公英黄酮类醇提物呈浓度依赖性增加上皮间质转化(EMT)相关蛋白E-cadherin的蛋白表达量,减少N-cadherin、Snail-1、ZO-1等蛋白的表达量。结论:蒲公英黄酮类醇提物显著抑制ESCC细胞增殖,并且通过阻遏EMT,进而抑制ESCC细胞迁移和侵袭能力,为蒲公英防治食管鳞癌提供有效实验依据。

Effects of Flavonoids Extract from Dandelion on the Proliferation and Invasion of Human Esophageal Squamous Cell Carcinoma

Objective:To study the effects of flavonoids from dandelion alcohol extract (dandelion flavonoids) on the proliferation, migration and invasion of human Esophageal squamous cell carcinoma (ESCC), and its potential mechanism. Methods:CCK-8 method was used to detect the effect of flavonoids extracted from dandelion on ESCC cells proliferation. Scratch assay and transwell migration assay were applied to observe the migration and invasion abilities of ESCCs after drug treatment. Western blot was used to investigate the mechanism of drug on ESCC cells migration and invasion. Results:The flavonoid extracted of dandelion significantly inhibited the proliferation of ESCC cells in a time-and dose-dependent manner, and remarkably blocked the abilities of migration and invasion of ESCC cells. Flavonoid extracts of dandelion increased the expression of EMT-related proteins E-cadherin and decreased the expression of N-cadherin, Snail-1 and ZO-1 proteins in a concentration-dependent manner. Conclusion:Dandelion flavonoids can significantly inhibit the proliferation, migration and invasion of ESCC cells. EMT pathway plays an important role in metastasis of flavonoids-treated ESCC cells. This study will provide an effective experimental basis for dandelion to prevent and treat esophageal squamous cell carcinoma.