超声法提取海南眼树莲总三萜的工艺优化及其抗氧化性

目的:优化眼树莲中总三萜的提取工艺,并研究其抗氧化能力。方法:采用超声方法提取眼树莲中三萜,并利用分光光度法测定其含量。首先利用单因素实验确定最初实验条件,在单因素基础上,使用L₃(3⁴)正交实验优化工艺。用DPPH·法测定优化后提取的总三萜抗氧化性。结果:4个因素对总三萜提取量影响大小为料液比 > 温度 > 超声功率 > 超声时间,最佳提取工艺为采用提取溶剂为乙酸乙酯,超声时间为30 min,料液比为1∶20 (g/mL),超声功率为200 W,温度为55 ℃,眼树莲中总三萜平均提取量为20.82 mg/g。该工艺提取获得的眼树莲总三萜浓度为2.00 mg/mL时,对DPPH·的清除率为26.86%,其抗氧活性低于V_C。结论:与传统溶剂提取、索氏提取相比,优化后的超声提取工艺提取眼树莲总三萜的提取量明显较高,平均提取量为20.82 mg/g,获得的眼树莲总三萜具有一定的抗氧化活性。

Optimization of Ultrasonic Extraction of Total Triterpenoids from Hainan Dischidia chinensis and Its Antioxidant Activity

Objective:To optimize the extraction process of total triterpenoids from Dischidia chinensis and to study the antioxidant capacity. Methode:Triterpenoids were extracted from Dischidia chinensis by ultrasound and their contens were determinated with spectrophotometry. Firstly, the initial test conditions were determined by single factor test. L₃ (34) orthogonal test was used to optimize the process based on single factor experiment. The antioxidant activity of total triterpenoids extracted after optimization by DPPH·.Results:The effects ability of four factors on the yield of total triterpenoids were as follows:Material-liquid ratio > temperature > ultrasound power > ultrasound time. The optimum extraction process was that the solvent was ethyl acetat, ultrasound time was 30 min, material-liquid ratio was 1:20 (g/mL), ultrasound power was 200 W, temperature was 55℃. And the average yield of total triterpenoids from Dischidia chinensis was 20.82 mg/g. When the concentration of total triterpenoids extracted by this process was 2.00 mg/mL, the DPPH removal rate was 26.86%, but its antioxidant activity was lower than that of V_C. Conclution:Compared with traditional solvent extraction and Soxhlet extraction, the yield of total triterpenoids extracted by the optimized ultrasonic extraction process was obviously higher, and the average yield was 20.82 mg/g. The obtained total triterpenoids from Dischidia chinensis had certain antioxidant activity.