天浆壳生物碱的提取分离纯化及对舌癌Tca8113细胞的增殖抑制作用

目的:从天浆壳中提取分离纯化得到生物碱,并研究其对人舌癌Tca8113细胞的增殖抑制作用。方法:采用醇提法提取天浆壳中总生物碱,硅胶柱色谱分离纯化得到单一生物碱化合物,通过核磁共振光谱和质谱对其化学结构进行分析。采用四甲基偶氮唑盐(MTT)比色法测定生物碱对Tca8113细胞增殖的抑制率,Hoechst 33342染色法观察细胞形态变化。结果:从天浆壳中分离出secu'amamine D、securinine、securitinine三种生物碱;MTT结果表明,三种生物碱均能明显抑制Tca8113细胞的增殖;Hoechst 33342染色结果显示,三种生物碱均能使Tca8113细胞发生凋亡形态学改变,且与浓度正相关。结论:首次从天浆壳中分离出secu'amamine D、securinine、securitinine三种生物碱,三种生物碱均能抑制Tca8113细胞增殖并促使其凋亡。

Extraction and Purification of Alkaloids from Fruit Shell of Metaplexis japonica (Thunb.) Makino and Its Inhibition of Proliferation of Human Tongue Cancer Tca8113 Cells

Objective:Extraction and purification alkaloids from fruit shell of Metaplexis japonica (Thunb.) Makino and its inhibition of proliferation of human tongue cancer Tca8113 cells were studied. Method:The total alkaloids from fruit shell of Metaplexis japonica (Thunb.) Makino was extracted by alcohols. Single alkaloid compound was isolated and purified by silica gel column chromatography.Its chemical structure was analyzed by NMR and MS. The inhibition rate of alkaloids on cell proliferation was determined by MTT assay colorimetry. Cell morphology was observed by Hoechst 33342 staining. Result:Three alkaloids, secu'amamine D, securinine and securitinine, were isolated from fruit shell of Metaplexis japonica (Thunb.) Makino. MTT results showed that three alkaloids could significantly inhibit the proliferation of Tca8113 cells. Hoechst 33342 staining showed that three alkaloids could induce apoptotic morphological changes in Tca8113 cells, and positively correlated with the concentration. Conclusion:For the first time, three alkaloids, secu'amamine D, securinine and securitinine, were isolated from fruit shell of Metaplexis japonica (Thunb.) Makino. The three alkaloids inhibited the proliferation of Tca8113 cells and promoted apoptosis.