实时PCR技术在羊亚科肉类鉴定中的应用

根据线粒体cyt b基因的种间差异,建立羊亚科、绵羊、山羊肉类的实时荧光聚合酶链式反应检测技术,对方法进行系统的优化与完善,并进行特异性、灵敏度与扩增效率、检出限、试剂一致性分析与适用性考察,同时邀请5家国内权威机构对方法进行复核。结果表明,方法的重复性好、适用性广,可用于不同基质的肉制品检测,扩增效率达95%以上,用于羊肉制品的动物源性成分检测准确率达100%,适于推广使用。

Application of Real-Time PCR Assay in the Identification of Caprinae Meat

A real-time polymerase chain reaction(real-time PCR)method for the identification of meat from Caprinae(including goats and sheep)was established and optimized based on the inter-species difference of cytochrome b(cyt b)gene.We evaluated the specificity,sensitivity,amplification efficiency,detection limit,reagent consistency and applicability of this method,and we invited five domestic authoritative institutions to validate it.The results showed that this method could be suitable for detecting Caprinae meat products with different substrates because of its good repeatability,wide applicability,high amplification efficiency(>95%)and accuracy(100%).