忧遁草多糖提取工艺优化及抗氧化活性研究

以提取温度、料液比和提取时间为影响因素,忧遁草多糖提取率为评价指标,采用Box-Behnken响应面法优化提取工艺。结果表明,忧遁草多糖的最佳提取工艺条件为料液比1:31 (g/mL),提取温度92℃,提取时间1.6h,此时多糖提取率为3.40%。抗氧化活性试验表明,忧遁草多糖具有良好的ABTS自由基和DPPH自由基清除效果,并具有一定的羟基自由基清除能力。

Optimization of extraction process and antioxidant activity of polysaccharide from Clinacanthus nutans by response surface methodology

The extraction temperature,ratios of water to material and extraction time were taken as the influencing factors,and the extraction rate of polysaccharides from Clinacanthus nutans was taken as the evaluation index to carry out single factor experiment,and Box-Behnken response surface methodology was used to optimize the extraction process on the basis of single factor test results.The results showed that the best extraction conditions of polysaccharides from Clinacanthus nutans were as follows:the ratio of material to liquid was 1:31(g/mL),the extraction temperature was 92 ℃,the extraction time was 1.6 h.and the extraction rate was 3.40%.The antioxidant activity test showed that the polysaccharide of Clinacanthus nutans had good scavenging ability in ABTS anion free radical and DPPH free radical and had certain scavenging ability on hydroxyl free radicals.