生物胺降解菌株的筛选与鉴定

从实验室保藏的不同来源乳酸菌菌株中筛选出具有生物胺降解能力的菌株,测定其在不同盐浓度、pH下的生长能力。结果表明,筛选出3株具有生物胺降解能力的菌株,经16S rDNA序列分析,菌株HM22鉴定为发酵乳杆菌(Lactobacillus fermentum),菌株HM24为植物乳杆菌(Lactobacillus plantarum),菌株YF10042为粪肠球菌(Enterococcus faecalis);其中植物乳杆菌HM24的降解能力最优,对色胺、苯乙胺、腐胺、尸胺、组胺、酪胺、亚精胺、精胺的降解率分别为(25.90±2.30)%,(35.75±2.71)%,(27.61±3.94)%,(25.91±3.76)%,(22.54±2.64)%,(34.55±1.90)%,(39.25±1.86)%,(55.66±7.08)%;当盐浓度为4%~10%时,3株菌株耐受力随盐浓度的增加而降低,;当pH为3.5~9.5时,3株菌株均可生长。

Screening and identification of biogenic amine degrading strains

Strains with biodegradability of biogenic amines were screened from lactic acid bacteria strains from different sources preserved in the laboratory, and their growth ability at different salt concentrations and pH values were determined. The results showed that three strains with the capability of degrading biogenic amines were screened out. Based on 16S rDNA sequence analysis, strain HM22 was identified as Lactobacillus fermentum, strain HM24 as Lactobacillus plantarum, and strain YF10042 as Enterococcus faecalis. Among them, Lactobacillus plantarum HM24 has the best degradation ability. The degradation rates of tryptamine, phenethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine are (25.90±2.30)%, (35.75±2.71)%, (27.61±3.94)%, (25.91±3.76)%, (22.54±2.64)%, (34.55±1.90)%, (39.25±1.86)%, (55.66±7.08)%, respectively. When the salt concentration is 4%~10%, the tolerance of 3 strains decreased with the increase of salt concentration; When pH is 3.5~9.5, all three strains can grow.