| 植物乳杆菌M1-NTG300产细菌素培养条件优化 |
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| 引用本文： | 安宇,王颖,易华西,张东杰.植物乳杆菌M1-NTG300产细菌素培养条件优化[J].食品与机械,2020(8):178-182,209. |
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| 作者姓名： | 安宇 王颖 易华西 张东杰 |
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| 作者单位： | 黑龙江八一农垦大学食品学院,黑龙江 大庆 163319;黑龙江八一农垦大学食品学院,黑龙江 大庆 163319 ；国家杂粮工程技术研究中心,黑龙江 大庆 163319;中国海洋大学食品科学与工程学院,山东 青岛 266000 |
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| 基金项目： | 国家重点研发计划项目(编号:2018YFE0206300)；国家“十二五”支撑计划项目(编号:2015BAD16B05) |
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| 摘 要： | 以植物乳杆菌素的效价作为特征性考察指标,对植物乳杆菌M1-NTG300产细菌素培养条件进行优化。通过响应面分析结合实际值确定培养基最佳组分为:葡萄糖浓度3.5%、蛋白胨浓度3.5%、吐温80浓度0.35%、氯化镁浓度0.20%,此培养条件下植物乳杆菌M1-NTG300所产细菌素的效价为1 269.4IU/mL,细菌素效价提高了19.8%。
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| 关 键 词： | 植物乳杆菌 细菌素 培养基 |
Optimizing conditions of producing bacteriocin by Lactobacillus plantarum M1-NTG300 |
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| AN Yu,WANG Ying,YI Hua-xi,ZHANG Dong-jie.Optimizing conditions of producing bacteriocin by Lactobacillus plantarum M1-NTG300[J].Food and Machinery,2020(8):178-182,209. |
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| Authors: | AN Yu WANG Ying YI Hua-xi ZHANG Dong-jie |
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| Abstract: | Taking the potency of phytolactin as a characteristic index, the culture conditions for bacteriocin production by Lactobacillus plantarum M1-NTG300 were optimized. Through response surface analysis combined with actual values, the optimal components of the culture medium were determined to include 3.5% glucose concentration, 3.5% peptone, 0.35% Tween 80, and 0.20% magnesium chloride. Under the control of these culture conditions, The titer of bacteriocin, produced by L. plantarum M1-NTG300 was 1 269.4 IU/mL, which was increased by 19.8%. |
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