黔产草果不同部位精油化学成分分析及体外抗氧化活性评价

采用水蒸气蒸馏法提取草果不同部位(花、根、茎叶)精油,利用气相色谱-质谱法对精油成分进行分析,经计算机质谱库和人工解析,应用保留指数法对化合物定性,峰面积归一化法计算相对含量,并比较不同部位精油体外抗氧化能力。结果表明,草果根精油提取率相对最高,达到0.79%。从草果花、根、茎叶精油中分别鉴定出26、25和34种化合物,占各自精油总量的98.87%、99.75%和97.56%。三个部位具有相同化合物19种,分别占各部位精油种类总量的90.7%、67.05%和80.79%。草果不同部位精油均具有一定的抗氧化能力,其中草果根精油对DPPH自由基的清除能力最高为(11.79±0.73) μmol Trolox/g;草果花精油对ABTS⁺自由基的清除能力最高为(112.96±1.22) μmol Trolox/g;草果茎叶精油对Fe³⁺的还原能力最高为(77.91±0.38) μmol Trolox/g。

Chemical Component Analysis and in Vitro Antioxidant Activity Evaluation of Essential Oils from Different Parts of Amomum tsaoko in Guizhou Province

The essential oils of different parts (flowers, root, leaves) of Amomum tsaoko were extracted by steam distillation. The components of essential oils were analyzed by GC-MS. Through computer mass spectrometry and manual analysis, the compounds were characterized by retention index method, and the relative contents were calculated by peak area normalization method, and the antioxidant capacity of different parts of essential oils in vitro was compared. The results showed that the extraction rate of essential oil reached 0.79%. 26, 25 and 34 compounds, accounting for 98.87%, 99.75% and 97.56% were identified from the essential oils of grass fruit flowers, roots and leaves. There were 19 compounds in the three parts, which accounted for 90.7%, 67.05% and 80.79% of the total number of essential oils in each part. The essential oils of different parts of grass fruit had certain antioxidant capacity, among which the highest scavenging capacity of grass fruit root essential oil on DPPH free radical was (11.79±0.73) μmol Trolox/g. The highest scavenging ability of the essential oil on ABTS+·was (112.96±1.22) μmol Trolox/g. The maximum reduction of Fe3+ by essential oils of stems and leaves was (77.91±0.38) μmol Trolox/g.