土鳖虫活性肽组分对急性血瘀模型大鼠血液流变学、血脂四项指标及血液因子含量的影响

用胃蛋白酶和胰蛋白酶复合酶系对土鳖虫药材进行仿生酶解,产物经除盐、超滤、纳滤、DA201-C型大孔吸附树脂、葡聚糖凝胶G-25层析、反相高效液相色谱以及半制备液相色谱分离纯化,获得纤溶活力最强的土鳖虫活性肽组分。并研究其对急性血瘀模型大鼠的血液流变学指标、血脂四项指标和血清内一氧化氮(NO)、超氧化歧化酶(SOD)、组织性纤溶酶原激活物(t-PA)和纤维蛋白(FIB)含量等的影响。结果表明:土鳖虫活性肽组分F2能显著降低血液黏度、红细胞压积、血小板计数(P<0.05);与模型组相比,土鳖虫活性肽组分F2能显著降低大鼠血液内甘油三脂(TG)、胆固醇(T-CHO)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的含量(P<0.05);大鼠血液中NO、SOD、t-PA含量较模型组明显升高,血清内FIB含量明显减少,差异有统计学意义(P<0.05)。土鳖虫经仿生酶解、分离纯化后制备的土鳖虫活性肽组分F2具有改善急性血瘀模型大鼠血液流变学指标,降低血脂四项指标和改变血液因子含量的作用。

Effects of active peptide components of Eupolyphaga steleophaga on blood rheology,four indicators of blood lipids and blood factor levels in rats with acute blood stasis

Pepsin and trypsin complex enzymes were used for biomimetic enzymatic hydrolysis of Eupolyphaga steleophaga.In order to obtain the most active fibrinolytic activity of the soil aphid active peptide component F2,the product was subjected to desalting,ultrafiltration,nanofiltration,DA201-C macroporous adsorption resin,dextran gel G-25 chromatography,reverse high performance liquid chromatography and semi-preparative liquid chromatography.Moreover,its effects on hemorheology index,blood lipids and serum nitric oxide(NO),superoxide dismutase(SOD),tissue plasminogen activator(t-PA)and fibrin(FIB)content of rats with acute blood stasis were also studied.E.steleophaga active peptide component F2 could significantly reduce blood viscosity,hematocrit and platelet count(P<0.05).Compared with the model group,the Eupolyphaga steleophaga active peptide component F2 can significantly reduce the content of triglyceride(TG),cholesterol(T-CHO),high-density lipoprotein(HDL)and low-density lipoprotein(LDL)m rat blood(P<0.05).The levels of NO,SOD and t-PA m the blood of rats were significantly higher than those in the model group,and sig-nificant differences in the levels of FIB between the experimental group and the model group(P<0.05)were also found.The E.steleophaga active peptide component F2 prepared by biomimetic enzymatic hydrolysis and purification had the functions of improving blood rheology index,reducing blood lipid levels and changing blood factor content in acute blood stasis model rats.