浓香型白酒发酵旺盛期糟醅中酵母菌菌群研究

充分解析并挖掘浓香型白酒糟醅中的酵母菌株资源,对五粮液发酵旺盛期中的糟醅进行高通量测序以及纯培养。其中通过高通量测序技术共检测出11个种属的酵母菌群,包括:Kazachstania exigua、Saccharomyces cerevisiae、Kazachstania humilis、Pichia kudriavzevii、Zygosaccharomyces bailii、Saccharomycopsis fibuligera、Trichosporon ovoides、Debaryomyces hansenii、Naumovozyma castellii、Pichia manshurica、Geotrichum silvicola。利用7种培养基筛选五粮液发酵旺盛期糟醅中的酵母菌株,通过合并共获得57株纯培养酵母菌株,通过26S rDNA序列鉴定,发现其分属于Pichia kudriavzevii、Saccharomyces cerevisiae、Candida glabrata和Candida rugosa。结果表明:浓香型白酒发酵旺盛期糟醅中的酵母群体多样性丰富,但与高通量测序相比,纯培养法仅能分离到糟醅酵母群体中的少数种属,因此要充分挖掘浓香型白酒糟醅中的酵母菌株资源,还需进一步优化分离、培养方法。

Study on the Yeast Group of Dregs in the Main Stage of Fermentation of Luzhou-flavor Liquor

In order to fully analyze and tap the yeast strain resources in the fermented grains of Luzhou-flavor liquor,high-throughput sequencing and pure culture were carried out for the fermented grains in the vigorous period of Wuliangye fermentation.Among them,a total of 11 species of yeast were detected by high-throughput sequencing technology,including Kazachstania exigua,Saccharomyces cerevisiae,Kazachstania Humilis,Pichia kudriavzevii,Zygosaccharomyces bailii,Saccharomycopsis fibuligera,Trichosporon ovoides,Debaryomyces hansenii,Naumovozyma castellii,Pichia manshurica,Geotrichum silvicola.The yeast strains in the fermented grains of Wuliangye at the vigorous fermentation stage were screened by 7 kinds of culture media.57 strains of pure culture yeast were obtained by merging.Through the sequence identification of 26 S rDNA,it was found that they belonged to Pichia kudriavzevii,Saccharomyces cerevisiae,Candida glabrata and Candida rugosa.The results showed the rich diversity of yeast population in fermented grains of Luzhou-flavor liquor.Nonetheless,compared with the high-throughput sequencing means,the pure culture method could only isolate a few species in the septic yeast population.Thus,it was necessary to dig out the yeast strain resources in the fragrant white wine dregs and further optimize the separation and culture methods.