翅果油对高脂饮食小鼠的降脂减肥作用

目的:研究翅果油干预对高脂饮食小鼠降脂减肥作用。方法:将40只C57BL/6小鼠随机分为5组(n=8):正常组、高脂饮食组、翅果油低、中、高剂量组,灌胃量分别为1.5、3.0和6.0 g/kg·d。正常组给予基础饲料,其余各组给予高脂饲料,干预8周。实验结束后记录小鼠体重、摄食量等基础指标,测定血清中总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、高密度胆固醇(High density lipoprotein cholesterol,HDL-C)及肝脏TC、TG的含量,并计算血清中低密度脂蛋白(Low density lipoprotein cholesterol,LDL-C)含量及动脉粥样硬化指数(Atherosclerosis index,AI),苏木精伊红染色(Hematoxylin and eosin,HE)染色观察脂肪组织细胞数量及大小,并进行相关性分析。结果:与高脂饮食组相比,翅果油干预组显著降低小鼠体重、脂肪重、肝重、肝脏TG含量及血清TC、TG、LDL-C含量(P<0.05),其中翅果油中剂量组干预效果最好;翅果油干预能增加血清HDL-C含量,降低AI值,但不具有显著性差异(P>0.05);HE染色结果显示,高脂饮食小鼠脂肪细胞变大且排列不均匀,翅果油干预能够抑制高脂饮食引起的脂肪细胞增大,且中剂量效果较好;相关性分析结果显示,脂肪细胞面积与肝脏TG、TC含量呈显著正相关,与血清HDL-C呈显著负相关,与AI值呈显著正相关(P<0.05),说明翅果油可以通过降低肝脏脂质含量,调节机体脂质代谢,从而抑制脂肪细胞增大,达到降脂减肥的作用。结论:翅果油干预可以在一定程度上有效预防肥胖及与肥胖相关的代谢疾病的发生。

The Prevention Effect of Elaeagnus mollis Oil on High-fat Diet Induced Obesity in Mice

Objective:The aim of this study was to investigate the decreasing effect of Elaeagnus mollis oil(EMO)on hyperlipidemia and obesity induced by high-fat diet. Method:Forty male C57BL/6 mice were randomly assigned into 5 groups(n=8):normal diet group,high-fat diet group and low-,middle- and high-dose EMO groups(gavaged with 1.5,3.0,6.0 g/kg·d of EMO,respectively). The mice in the normal diet group were fed with normal diet,while the remaining groups were fed with high-fat diet for 8 weeks. Body weight and food intake were monitored throughout experiment. At the end of study,the levels of serum TC,TG,HDL-C and hepatic TC,TG were measured. The content of serum LDL-C and AI value were calculated,and histological examinations were conducted to analysis the number and size of adipose tissue cells. Meanwhile,the correlation analysis was performed. Results:The results showed that Elaeagnus mollis oil significantly reduced body weight,fat weight,liver weight,Lee's index,hepatic TG level,serum TG,TC and LDL-C levels compared with HFD group(P<0.05),especially the middle-dose EMO group. The supplement of EMO increased the level of HDL-C and decreased AI value,but no significant change was observed(P>0.05). Moreover,the result of HE staining showed that adipocyte became larger and disordered on account of high-fat diet,while supplementing EMO effectively blocked adipocytosis,and the improving efficiency was more prominent of middle-dose EMO group. Correlation analysis showed that adipocyte hypertrophy displayed a positively correlated with hepatic TG and TC,a negatively correlated with serum HDL-C and a positively correlated with AI(P<0.05),which suggested that Elaeagnus mollis oil could reduce lipid content in liver and regulate lipid metabolism to inhibit adipocytosis,and achieved the aim of reducing hyperlipidemia and obesity. Conclusion:Elaeagnus mollis oil can be beneficial as a functional food to prevent obesity and other obesity-related metabolic diseases.