山药多糖提取工艺的响应面法优化及其功能活性分析

利用Box-Behnken设计-响应面优化山药多糖的提取工艺,初步评价不同产地山药多糖清除1,1-二苯基-^2-三硝基苯肼(DPPH·)能力、羟自由基(OH·)能力和超氧阴离子(O^2-·)能力和α-葡萄糖苷酶抑制活性能力。以超声辅助提取温度、提取时间和料液比为自变量,以山药多糖提取率为因变量,采用响应面分析法优化山药多糖超声辅助提取工艺:提取温度66℃,提取时间26 min,液料比22∶1(mL/g),在此条件下,多糖得率为9.34%。不同产地山药多糖对α-葡萄糖苷酶活性具有抑制作用,并随着多糖浓度的提高其抑制率随之提高。抗氧化活性试验表明山药多糖对DPPH·、OH·和O^2-·具有较显著清除作用,并呈现一定的浓度依赖性。其中4个产地山药多糖中河南怀山药多糖含量最高,对α-葡萄糖苷酶的抑制作用、对DPPH·和OH·自由基的清除能力均最优,预处理河南为山药道地产区质优效佳的传统认知相符。

Optimization of Extraction Technology for Chinese Yam Polysaccharides by Response Surface Method and Its Hypoglycemic and Antioxidant Activity

To optimize the extraction technology for Chinese yam polysaccharides by response surface method,and preliminary researched the DPPH free radical scavenging capacity(DPPH·),hydroxyl radical(OH·),superoxide anion radical(O^2-·)and alpha glucosidase inhibitory activity.In order to evaluate the indicators,the effects of ultrasonic extraction temperature,extraction time and ratiliquid-solid ratio on Chinese yam polysaccharide yield were compared by Box-Behnken design-response surface method.The optimal ultrasonic extraction technology were determined as follows:extraction temperature of 66℃,extraction time for 26 min,liquid-to-material ratio of 22∶1(mL/g),under which the yield of polysaccharide was 9.34%.Polysaccharides from different habitats have an inhibitory effect on alpha glucosidase activity,and the inhibition rate increases with the increase of polysaccharide concentration.The antioxidant activity test showed that polysaccharide had a significant scavenging effect on DPPH·,OH·and O^2-·,and showed a certain concentration dependence.The content of polysaccharides in Henan province was the highest,the inhibitory effects on alpha glucosidase activitie and the scavenging activities of DPPH·and OH·were significant.