菊苣、葛根、桑叶配方对高尿酸合并关节炎小鼠的降尿酸和痛风性关节炎的干预效果

目的:探究菊苣、葛根、桑叶配方的潜在降尿酸和抗痛风作用及机制。方法:50只KM雄性小鼠随机分为空白、模型、别嘌醇阳性(5 mg·kg-1)、配方高剂量(2.67 g·kg-1)、配方低剂量组(1.33 g·kg-1),每组10只。氧嗪酸钾以0.1 mL/10 g剂量腹腔注射造模,1 h后,配方以0.1 mL/10 g剂量灌胃给药,PO造模的第9 d,以尿酸钠晶体混悬液(25 mg·mL-1)诱导痛风关节炎,每2 d注射1次,连续15 d。检测血清中炎症因子、尿酸(UA)、黄嘌呤氧化酶(XOD)以及肝肾功能指标,Western Blot方法检测尿酸盐转运体(URAT1)的表达。结果:与模型组相比,配方高、配方低剂量组均可降低小鼠关节炎症指数,其关节红外温度、血清UA、TNF-α、CRE、BUN、AST、ALT水平均有显著或极显著降低(P<0.05或P<0.01);配方高剂量组小鼠的关节肿胀度和炎症因子IL-1β均显著降低(P<0.05),XOD水平极显著降低(P<0.01);关节肿胀程度有所降低,肝肾组织形态有所改善;肾脏组织中URAT1蛋白水平显著下降(P<0.05)。结论:菊苣、葛根、桑叶配方具有降尿酸和抗痛风作用,其作用机制可能与改善肝肾损伤,降低血清尿酸以及减少肾脏组织中URAT1蛋白的表达相关。

Intervention Effects of Chicory,Pueraria and Mulberry Leaf Formula on Reducing Uric Acid and Gouty Arthritis in Mice with High Uric Acid and Arthritis

objective:In order to investigate the potential uric acid lowering and anti-gout effect and mechanism of the formulation of chicory,pueraria and mulberry leaves. Methods:50 KM male mice were randomly divided into normal,model,allopurinol-positive(5 mg·kg-1),high-dose(2.67 g·kg-1)and low-dose(1.33 g·kg-1)groups,with 10 mice in each group. Potassium oxonate was injected intraperitoneally at a dose of 0.1 mL/10 g to make the model mice. One hour later,the formula was gavage at a dose of 0.1 mL/10 g. On day 9 of the model,sodium urate crystal suspension(25 mg·mL-1)was used to induce gout arthritis. Inflammatory factors,uric acid(UA),xanthine oxidase(XOD)as well as liver and kidney function indexes in serum were detected,and the expression of urate transporter(URAT1)was detected by Western Blot. Results:Compared with the model group,both the high and low dose groups could reduce the index of joint inflammation in mice,and the level of joint infrared temperature,serum UA,TNF-α,CRE,BUN,AST and ALT were significantly decreased(P<0.05 or P<0.01).The degree of joint swelling and IL-1β level were decreased in the high-dose group(P<0.05),and the XOD level was significantly decreased(P<0.01). The degree of joint swelling was decreased and the morphology of liver and kidney tissues was improved. And the expression of URAT1 protein in kidney tissues was decreased(P<0.05). Conclusion:The formulations of chicory,pueraria and mulberry leaves possessed the functions of lowering uric acid and anti-gout,the mechanism of which may be related to improving liver and kidney injury,lowering serum uric acid and reducing the expression of URAT1 protein in kidney tissues.