响应面法优化超声微波联合提取杏鲍菇中麦角硫因工艺

为优化杏鲍菇中麦角硫因的提取工艺,以麦角硫因含量为指标,研究干燥方式、液料比、乙醇浓度、微波条件和超声条件等单因素对提取杏鲍菇中麦角硫因的影响,并在单因素实验的基础上使用响应面法优化杏鲍菇中麦角硫因的提取工艺。结果表明:真空冷冻干燥后杏鲍菇中麦角硫因含量为(0.20±0.02)mg/g湿重,显著高于热风干燥所得的(P<0.05),但与新鲜杏鲍菇的没有显著性差异(P>0.05),这表明真空冷冻干燥处理能很好地保留杏鲍菇中的麦角硫因;结合单因素试验和响应面分析,确定超声微波联合提取杏鲍菇中麦角硫因的最佳工艺条件为液料比48:1(mL/g)、53%乙醇、微波条件(500 W,65℃,5 min)、超声条件(450 W,5 min),在此优化条件下,获得麦角硫因的含量为(3.79±0.02)mg/g干重,与预测值误差在1%以内。本研究所得到的优化工艺条件具有一定的可行性,可为食用菌中麦角硫因的提取提供参考,具有广阔的应用前景。

Optimization of Ultrasonic Combined Microwave-assisted Extraction of Ergothioneine in Pleurotus eryngii by Response Surface Methodology

In order to optimize the extraction process of ergothioneine in Pleurotus eryngii,the effects of drying method,liquid to material ratio,ethanol concentration,microwave conditions and ultrasonic conditions on the extraction of ergothioneine in Pleurotus eryngii were studied with the content of ergothioneine as indicator.And the response surface methodology which was based on the single factor experiment was conducted to obtain the optimum formulations of extraction of ergothioneine in Pleurotus eryngii.The results showed that the content of ergothioneine in Pleurotus eryngii was(0.20±0.02)mg/g wet weight after vacuum freeze-drying,which was significantly higher than that obtained by hot air drying(P<0.05),but it was not significant difference with fresh Pleurotus eryngii(P>0.05).This indicated that the vacuum freeze-drying treatment could well retain the ergothioneine in Pleurotus eryngii.The optimum conditions for the extraction of ergothioneine in Pleurotus eryngii by ultrasound combined microwave were as follows:Liquid to material ratio 48∶1(m L/g),53%ethanol,microwave conditions(500 W,65℃,5 min),ultrasonic conditions(450 W,5 min).Under this optimized condition,the extraction of ergothioneine was(3.79±0.02)mg/g DW,and the error with the predicted value was within 1%.The optimized process conditions obtained in this study had certain feasibility,which could provide reference for the extraction of ergothioneine in edible fungi,and had broad application prospects.