石斛与粉葛体外体内降糖降脂协同作用研究

以阿卡波糖作对照,分析了石斛粉葛咀嚼片中石斛与粉葛对α-葡萄糖苷酶抑制活性的协同作用;以黑檀体果蝇为试验动物,建立高糖及高脂诱导的果蝇2型糖尿病模型,石斛组、粉葛组、石斛粉葛组分别给药7d后,以总蛋白、甘油三酯、海藻糖含量为考察指标,研究各试验组对果蝇糖脂代谢的影响,同时分析了粉葛和石斛的协同降糖降血脂作用。结果表明:水提液、醇提液各组对α-葡萄糖苷酶抑制作用均呈剂量—效应关系,水提液抑制作用从强到弱顺序为:阿卡波糖>石斛咀嚼片水提液>石斛粉葛咀嚼片>粉葛咀嚼片水提液,以石斛咀嚼片抑制作用最强,而醇提液以粉葛咀嚼片作用最强,石斛与粉葛无显著的体外降糖协同作用;与模型组相比,石斛粉葛组降低甘油三酯(P<0.01)及海藻糖(P<0.05)的效果最佳,石斛和粉葛在体内有协同降糖降脂作用。

Synergistic effects of Dendrobium and Pueraria thomsonii on hpyerglycemic and lipid-lowing between in vitro and in vivo

The synergistic effects were observed on inhibiting al-pha-glucosidase activity between Dendrobium and Puerana thomsonii form self-made chewable tablets by taking the acarbose as control.Drosophila melanogaster was used as experimental animal,and the model ofⅡdiabetic Drosophila melanogaster was prepared by high glucose and high fat feeding.The experiment was divided into model control group,positive control group,Dendrobium(D)group,Pueraria thomsonii(PT)group and(Dendrobium+Pueraria thomsonii)(D+PT)group.After 7 days of lipid-lowering effects between Dendrobium and Pueraria thomsonii was analyzed,results showed that all of groups dose-effect perfusion,the total protein,triglycerides and trehalose content were measured.Compared with control group,the influence of experiments on glycolipid metabolism in Drosophila melanogaster was researched,respectively.Furthermore,the Dendrobium and Pueraria thomsonii showed synergistic relationship on inhibition of alpha-glucosidase activity.The results showed that each groups of water extract and alcohol extract played a certain role in inhibition of alpha-glucosidase activity with dose-effect relationship,and the ranking of inhibition effect of water extract groups was acarbose>D〉(DH-PT)>PT,and among which D group showed strongest effect while PT group was the strongest in the alcohol extract.Therefore,D and PT had no synergistic effects in vitro.It was found that D group,PT group and(D+PT)group had the effect of triglycerides and trehalose lowering,but had no significant effect on total protein content(P>0.05).Compared to the model group,the the(D+PT)group hand the best effect on lowing Lriglycendes(P<0.05)and trehalose(P<0.01).It is indicated that Dendrobium and Pu-erarta thomsonii had synergistic effeca in vivo.