神农架林区中蜂蜜理化指标及抗氧化活性分析

该研究以湖北神农架林区5个中蜂蜜样品为研究对象,对其理化指标和总酚酸、总黄酮含量进行测定,采用DPPH·和ABTS+自由基清除试验测定其抗氧化活性。试验结果表明,神农架林区5个中蜂蜜样品理化指标均符合GH/T 18796-2012《蜂蜜》,总黄酮含量为(9.95±0.04)mg/100 g^13.66±0.04mg/100 g;总酚酸含量为(20.69±0.03)mg/100 g^31.71±0.07mg/100 g;5个中蜂蜜样品均具有DPPH·和ABTS+自由基清除能力,IC50值分别在(39.14±0.16)mg/mL^106.63±0.38mg/mL和(48.48±0.22)mg/mL^127.07±1.41mg/mL之间。其中总酚酸含量与清除DPPH·和ABTS+自由基能力存在显著正相关,其相关系数分别为0.973、0.920。该研究首次报道了神农架林区中蜂蜜的理化性质及其抗氧化活性,为神农架林区中蜂蜜的开发利用提供了理论依据。

Analysis of Physicochemical Characterization and Antioxidant Activity of Honey Produced by Apis cerana in Shennongjia Forestry District

Five honey varieties from Shennongjia forestry district in Hubei were assessed including physical and chemical indicators,contents of total phenolics and total flavonoid.Their antioxidant activities were determined by DPPH·and ABTS+free radical scavenging experiments.The results showed that the physical and chemical indicators of five honey varieties from Shennongjia forestry district were confromed with honey.The contents of total flavonoids ranged from(9.95±0.04)mg/100 g to(13.66±0.04)mg/100 g.The contents of total phenolic acids ranged from(20.69±0.03)mg/100 g to(31.71±0.07)mg/100 g.The five honey varieties were identificated DPPH·and ABTS+free radical scavenging capacity with IC50 of(39.14±0.16)mg/mL to(106.63±0.38)and(48.48±0.22)mg/mL to(127.07±1.41)mg/mL.The total phenolics content was positively correlated with DPPH·and ABTS+free radical scavenging ability,and the correlation coefficients was 0.973 and 0.920,respectively.It was reported the physical and chemical indicators and antioxidant activities of honey in Shennongjia forestry district for the first time in this study,which provided a theoretical basis for the development and utilization of honey in Shennongjia forestry district.