超声波辅助低共熔溶剂提取野菊花总黄酮的工艺研究

目的:采用一种新型溶剂提取野菊花总黄酮,并对其提取工艺进行考察。方法:以野菊花总黄酮得率为指标,采用单因素实验和响应面试验优化野菊花总黄酮的提取工艺。结果:用摩尔比1:3的氯化胆碱和1,4-丁二醇制备低共熔溶剂,当低共熔溶剂含水量为28%,料液比1:25 g/mL,温度65 ℃下超声(功率450 W)提取38 min时,总黄酮得率可达62.16 mg/g。结论:低共熔溶剂可作为一种新型的溶剂高效提取野菊花中总黄酮。

Ultrasound-assisted Deep Eutectic Solvent Extraction of Total Flavonoids from Chrysanthemum indicum

Objective:To investigate the extraction of total flavonoids from Chrysanthemum indicum using a novel solvent. Methods:The extraction yield of total flavonoids from Chrysanthemum indicum acted as an index.The single factor experiment and response surface experiment were carried out to investigate the extraction of total flavonoids. Results:The optimized extraction conditions were as follows:A deep eutectic solvent was prepared by choline chloride and 1,4-butanediol in a molar ratio of 1:3,28% water in deep eutectic solvent,material to liquid ratio of 1:25 g/mL,ultrasonic power of 450 W,ultrasonic temperature of 65 ℃ and ultrasonic time of 38 min,the extraction rate of total flavonoids reached 62.16 mg/g. Conclusion:The deep eutectic solvent could be used as a new type of solvent to efficiently extract the total flavonoids from Chrysanthemum indicum.