魔芋寡糖的膜分离及产物组成分析

本文采用高速离心、无机陶瓷微孔滤膜、超滤纳滤对250 g/L(w/v)的高浓度魔芋精粉的酶解液进行分离、过滤、浓缩、制备魔芋寡糖,分析了甘露聚糖酶水解催化高浓度魔芋粉形成的具有一定粘性的寡糖溶液,对过滤通量、寡糖的回收率和寡糖产物组成的影响。结果表明,150 g/L的寡糖混合物通过超滤膜过滤,寡糖回收率能达到86%以上;组合纳滤膜能使寡糖混合物进行有效浓缩,可以去除溶液中的金属离子、有机酸小分子和溶剂水,提高寡糖溶质纯度。100~5000 Da的魔芋寡糖混合物通过基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)分析发现,寡糖的构成比例中三糖为最高单一组分,含量为21.5%,二糖~十四糖的总含量达到90%,十五糖-二十糖的含量为9%。因此,采用膜过滤技术用于魔芋寡糖分离、提纯和浓缩工艺是可行的。

Membrane Separation and Product Composition Analysis of Konjac Oligosaccharides

Centrifuge,micro porous filter membrane,and ultrafiltration-nanofiltration were used to separate,filter,and concentrate the enzymatic hydrolysis solution of konjac for preparing konjac oligosaccharides. Soluble glycan filtrates of 150 g/L filtered through the ultrafiltration membrane and the oligosaccharides recovery rate could reach over 86%. The filtration flux of the nanofiltration membrane decreased with the concentration of soluble glycan increasing. The matrix-assisted laser desorption ionization-time of flight mass spectrometer(MALDI-TOF-MS)used to analyze 100~5000 Da konjac oligosaccharides mixtures. The results showed that the content of trisaccharide was the highest single component with 21.5%,the total content of disaccharides to(C₆H₁₀O₅)₁₄ reached 90%,and(C₆H₁₀O₅)₁₅~(C₆H₁₀O₅)₂₀content was 9%. It was feasible to use membrane filtration technology in the separation,purification and concentration of konjac oligosaccharides.