动态高压微射流辅助提取对鱼鳞多糖抗氧化活性的影响

采用动态高压微射流技术辅助提取鲤鱼鱼鳞多糖,以DPPH·、·O₂^-、ABTS⁺·、·OH清除能力和Fe³⁺还原能力为评价指标,研究其对鲤鱼鱼鳞多糖抗氧化活性的影响。结果表明:动态高压微射流处理可以有效降低物料的平均粒度,粒径由未经处理的499.80 nm降低到192.57 nm;进而提高鲤鱼鱼鳞多糖的得率,当处理压力为130 MPa时,多糖得率达到7.81%。随着处理压力的提高,多糖提取液对DPPH·、·O₂^-、ABTS⁺·和·OH的清除率以及Fe³⁺的还原能力逐渐增强,当处理压力为110 MPa时,DPPH·、·O₂^-、·OH的清除率和Fe³⁺的还原能力最高分别达到了86.04%、61.23%、90.59%和0.64(OD值),当处理压力为150 MPa时,对ABTS⁺·的清除率达到89.58%,均显著高于空白对照组(P<0.05)。动态高压微射流辅助提取可以有效提高鲤鱼鱼鳞多糖的得率和体外抗氧化能力,本研究可为动态高压微射流技术在天然活性多糖辅助提取方面的应用提供理论依据和技术支撑。

Effects of Dynamic High Pressure Microfluidization-assistant Extraction on the Antioxidation of Polysaccharide from Carp Scales

Dynamic high pressure microfluidization was used for assistant extraction of polysaccharide from carp scales, and DPPH·,·O₂-, ABTS+·,·OH scavenging power and Fe3+ reducing power were used to investigate the antioxidant activities of polysaccharide. The results indicated that the average particle size of carp scales powder which were treated with dynamic high pressure microfluidization was decreased from 499.80 nm to 192.57 nm. The yield of total polysaccharide was also greatly increased, the extraction yield was up to 7.81%, when the pressure of treatment was 130 MPa. With the increasing of pressure, DPPH·scavenging activity,·O₂- scavenging activity, ABTS+·scavenging activity,·OH scavenging activity and Fe3+ reducing power were increasingly enhanced. The highest value of DPPH·scavenging activity,·O₂- scavenging activity,·OH scavenging activity and Fe3+ reducing power were up to 86.04%, 61.23%, 90.59% and 0.64 (OD value), respectively, when the pressure of treatment was 110 MPa. The highest value of ABTS+·scavenging activity was up to 89.58%, when the pressure of treatment was 150 MPa. All treatments were significantly higher than the control group (P<0.05). The extraction yield and antioxidant activity of polysaccharide from carp scales was improved by dynamic high pressure microfluidization-assistant extraction. This study could provide the theoretical basis and technical support for the application of dynamic high pressure microfluidization in natural active polysaccharide extraction.