流动注射化学发光法在线监测城市饮水中毒黄素突发性污染

基于碱性条件下毒黄素对鲁米诺-过氧化氢-纳米氧化铜化学发光体系光强的显著抑制作用,建立了一种在线监测和预警新方法。结合流动注射分析手段,通过微弱发光测量仪测定不同混合溶液光强。对4个影响因素进行单因素试验得出最佳结果,在此基础上经响应面优化各试验条件后进行标准曲线绘制及检出限测定,最后对不同水样进行加标回收。结果表明,该方法线性范围0.005~5.000mg/L,检出限0.001mg/L,加标回收率84%~114%。对浓度0.05 mg/L毒黄素平行测定11次,相对标准偏差(RSD)0.28%。该方法快捷便利,操作性强,可应用于城市饮水中毒黄素潜在突发性污染的快速在线监测和预警。

On-line monitoring the emergency pollution of toxoflavin in the municipal drinking water by flow injection chemiluminescence

Based on chemiluminescence inhibition effect of toxoflavin on luminol-hydrogen peroxide-copper oxide nanoparticles system in alkaline medium, a novel method for monitoring toxoflavin potential emergency pollution in municipal drinking water was successfully developed. Combining with flow injection method, the chemiluminescence intensity of different mixed solutions was determined by ultra-weak chemiluminescence analyzer. Optimal conditions were obtained by the means of one-way tests among 4 factors. Based on the one-way tests result, a response surface experiment was conducted in order to acquire standard curve, detection limit and recovery of different water samples. The linear range for toxoflavin monitoring was 0.005~5.000 mg/L with a detection limit (LOD) of 0.001 mg/L and recovery of 84%~114%. The relative standard deviation (RSD) of addition of 0.05 mg/L toxoflavin was 0.28% (n=11). This method was rapid, convenient, operable and could be applied to on-line monitoring and emergency warning of potential emergency pollution of toxoflavin in municipal drinking water.