黑木耳花青素提取工艺的优化及其抗氧化活性研究

为探讨黑木耳中花青素类物质最优提取工艺,以花青素提取率为考察指标,以提取试剂、温度、时间和液料比为提取因素,单因素试验基础上设计正交优化试验,研究黑木耳中花青素的最佳提取工艺;进一步利用大孔树脂对花青素粗提物进行纯化,纯化产物经自由基清除试验和抗菌试验等评价其生理学活性。结果表明,黑木耳花青素最佳提取条件为:提取剂酸化乙醇、液料比20∶1(mL/g)、温度60℃、时间60 min,且花青素提取物的抗氧化能力明显高于等浓度抗坏血酸;同时黑木耳花青素提取物对大肠杆菌和金黄色葡萄球菌具有较好的抑制作用。

Extraction and Antioxidant Activity Research of Auricularia auricula Anthocyanin

In order to investigate the optimal extraction of Auricularia auricula. Based on the anthocyanin extraction rate,extracting reagent,operating time,operating temperature,and the ratio of material to solution were studied. Orthogonal experiments were designed base on singly factor experiments to obtain the optimum extraction process. Macroporous resin was used to purify the crude extracts. The extracts were evaluated for their antioxidant activity and antioxidant activity. The results showed that the optimal conditions of anthocyanin extracts were as follows:solid-liquid ratio 20 ∶ 1(mL/g),incubation temperature 60 ℃,incubation time 60 min,and acidify ethanol as extractants. Further,the antioxidant capacity was significantly higher than the same concentration of ascorbic acid. And the anthocyanin extracts had significant inhibition effect on the growth of Escherichia coli and Staphylococcus aureus.