香芋皮多酚的超声提取工艺优化及其抗氧化活性

为了对香芋进行高效利用,以香芋加工副产物香芋皮为原料,采用超声波辅助提取香芋皮多酚,在单因素试验基础上结合正交试验方法对香芋皮多酚提取条件进行优化,并初步评价其体外抗氧化活性。结果表明,香芋皮多酚最佳的提取工艺为:超声功率400 W,乙醇浓度45%,料液比1:55 g/mL,超声时间35 min,此条件下香芋皮中多酚得率达到(23.61±0.36)mg/g。抗氧化活性实验表明,在最佳条件下提取得到的香芋皮多酚具有一定的还原能力,20.0 μg/mL的香芋皮多酚还原能力为0.4431±0.0027,并且其对DPPH自由基和ABTS自由基清除作用均表现出良好的清除效果,相应的IC₅₀值分别为(5.6647±0.2545)和(36.3630±2.4013)μg/mL,说明香芋皮多酚具有较强的抗氧化活性。

Optimization of Ultrasonic Extraction of Polyphenol from Taro (Colocasia esculenta)Peels and Its Evaluation of Antioxidant Activity

In order to utilize taro(Colocasia esculenta) efficiently,the taro peels were used as raw material to extract by ultrasound-assisted. On the basis of single factor experiment,orthogonal experiment was used to optimize the extraction conditions of polyphenols in taro peels and its antioxidant activity in vitro was preliminarily evaluated. The results showed that the optimum extraction process was as follows:Ultrasonic power 400 W,ethanol concentration 45%,solid-liquid ratio 1:55 g/mL,ultrasonic time 35 min. The polyphenol content in the taro peels extracted under this condition was(23.61±0.36) mg/g. Antioxidant activity experiments showed that the polyphenols extracted from taro peels had reduction ability,and the 20.0 μg/mL taro peels polyphenol had a reduction ability of 0.4431±0.0027. The IC₅₀ values of DPPH and ABTS scavenging activities were (5.6647±0.2545) and (36.3630±2.4013) μg/mL. The results indicated that polyphenols from taro peels exhibited positive antioxidant capacity.