| 产右旋糖酐肠膜明串珠菌发酵培养基的优化 |
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| 引用本文： | 张九花,刘祥芳,黎志德,梁达奉,吴兆鹏,李雨虹,黄曾慰.产右旋糖酐肠膜明串珠菌发酵培养基的优化[J].食品工业科技,2020,41(3):146-152. |
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| 作者姓名： | 张九花 刘祥芳 黎志德 梁达奉 吴兆鹏 李雨虹 黄曾慰 |
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| 作者单位： | 1. 广东省生物工程研究所(广州甘蔗糖业研究所), 中国轻工业甘蔗制糖工程技术研究中心, 广东省酶制剂与生物催化工程技术研究中心, 广东广州 510316;2. 广东工业大学轻工化工学院, 广东广州 510006 |
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| 基金项目： | 广东省科学院项目(2019GDASYL-0105032)。 |
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| 摘 要： | 旨在提高肠膜明串珠菌Leuconostoc mesenteroides CICC-23614发酵蔗糖产右旋糖酐的效率。以单因素实验为基础,以蔗糖、细菌蛋白胨、Na2HPO4·12H2O及KH2PO4浓度作为影响因子,以蔗糖转化率为响应值,采用响应面分析法对肠膜明串珠菌培养基中各成分浓度条件进行研究,利用高效液相色谱法(HPLC)对发酵液中的蔗糖进行定量分析,优化得到最佳发酵培养基。结果显示,经过优化的最佳的培养基为:蔗糖101.31 g/L,细菌蛋白胨5.66 g/L,Na2HPO4·12H2O 1.11 g/L和KH2PO40.15 g/L。以优化后的培养基进行发酵,重复试验验证,发酵24 h,蔗糖转化率可达91.9%,与预测值误差仅为2.13%,相较于原始培养基发酵结果,蔗糖转化率是原始培养基培养条件下的1.6倍。
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| 关 键 词： | 肠膜明串珠菌 高效液相色谱分析(HPLC) 蔗糖转化率 响应面分析 |
| 收稿时间： | 2019-05-14 |
Optimization of Fermentation Medium for Producing Dextran from Leuconostoc mesenteroides |
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| ZHANG Jiu-hua,LIU Xiang-fang,LI Zhi-de,LIANG Da-feng,WU Zhao-peng,LI Yu-hong,HUANG Zeng-wei.Optimization of Fermentation Medium for Producing Dextran from Leuconostoc mesenteroides[J].Science and Technology of Food Industry,2020,41(3):146-152. |
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| Authors: | ZHANG Jiu-hua LIU Xiang-fang LI Zhi-de LIANG Da-feng WU Zhao-peng LI Yu-hong HUANG Zeng-wei |
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| Affiliation: | 1. Guangdong Province Enzyme Preparation and Biocatalysis Engineering Technology Research Center, Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangdong Provincial Bioengineering Institute(Guangzhou Sugarcane Industry Research Institute), Guangzhou 510316, China;2. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China |
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| Abstract: | This paper aimed to improve the fermentation efficiency of dextran production with Leuconostoc mesenteroides CICC-23614. Based on the single factor optimization experiment,taking concentration of sucrose,peptone,Na2HPO4·12H2O and KH2PO4 as the influencing factors and the sucrose conversion rate as response value,the concentration conditions of various components in the culture medium of Leuconostoc mesenteroides were investigated by response surface experiment. The sucrose concentration was analyzed by high performance liquid chromatography. The results showed that the optimal medium components were sucrose of 101.31 g/L,peptone of 5.66 g/L,Na2HPO4·12H2O of 1.11 g/L and KH2PO4 of 0.15 g/L. Through verified trials with optimized medium,the sucrose conversion rate was 91.9% after 24 h of fermentation,the error with the predicted value was only 2.13%,which was 1.6 times than that of the control medium. |
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