Detection of roe deer,red deer,and hare meat in raw materials and processed products available in Poland |
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Authors: | Lech Rak Karina Knapik Jacek Bania Jakub Sujkowski Adam Gadzinowski |
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Affiliation: | 1. Department of Food Hygiene and Consumer Health Protection, Wroc?aw University of Environmental and Life Sciences, Wroc?aw, Poland
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Abstract: | To allow detection of meat from the most popular game species in Poland, we developed a PCR-based method for identification of roe deer (Capreolus capreolus), red deer (Cervus elaphus), and hare (Lepus europaeus). The designed primers were based on the noncoding, mitochondrial D-loop region. Amplicon sizes ranged from 116 to 255 bp. The primers exhibited no cross-reactivity with the DNA from common slaughter and other game species. The detection limit of the assay was established to be below 0.001 % in raw red deer (C. elaphus) and hare (L. europaeus) meat, and below 0.01 % in raw roe deer (C. capreolus) meat, whereas <0.5 % of hare and red deer meat in processed samples could be detected. The PCR-based assay was used for authentication of 17 samples of raw game meat and 32 samples of game meat-containing products available in Polish markets. Analysis of all tested raw meat and processed products revealed the presence of DNA of investigated species in concordance with producers’ declarations. |
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