| 黑曲霉固态发酵生产果胶酶培养条件的研究 |
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| 引用本文： | 冯红,;刘晓兰,;郑喜群.黑曲霉固态发酵生产果胶酶培养条件的研究[J].齐齐哈尔轻工业学院学报,2008(5):61-64. |
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| 作者姓名： | 冯红 ;刘晓兰 ;郑喜群 |
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| 作者单位： | [1]齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔161006; [2]齐齐哈尔大学农产品加工黑龙江省普通高校重点实验室,黑龙江齐齐啥尔161006 |
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| 基金项目： | 黑龙江省自然科学基金资助项目（C2004-34）;教育部科学技术研究重点项目（208038） |
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| 摘 要： | 对黑曲霉HYA4固态发酵生产果胶酶的培养条件进行了研究，固态发酵培养基组分为：250mL三角瓶中甜菜渣5g，黄豆粉5g，NH4H2PO4 0．4％，FeSO4·7H2O 0．05％．料水比1：2。采用此优化的培养基在培养温度36℃和初始pH自然条件下进行发酵。果胶酶酶活力可达5465．8U／g（干曲）。
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| 关 键 词： | 黑曲霉 甜菜渣 固态发酵 果胶酶 |
Optimizations of solid state fermenting condition of pectinase by Aspergills niger HYA4 |
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| Affiliation: | FENG Hong, LIU Xiao-lan, ZHENG Xi-qun ( 1. College of Life Sciences and Engineering, Qiqihar University, Heilongjiang Qiqihar 161006, China; 2. Key Laboratory of Processing Agricultural Products of Heilongjiang Province,Qiqihar University,Heilongjiang Qiqihar 161006, China ) |
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| Abstract: | Peetinase processing condition by solid state fermentation with Aspergills niger HYA4 as strain was studied. The optimum compositions of medium were： beet pomace 5 g, soybean power 5 g, NH4H2PO4 0.4%, FeSO4· 7H2O 0.05%, proportion of material and water 1 ： 2. Under the above culture conditions in 250 mL erlenmeyer flask, the enzyme activity of pectinase was increased to 5 465.8 units per dry koji. |
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| Keywords: | Aspergillsniger beet pomace solid fermentation pectinase |
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