Metabolism of platelet-activating factor by blood platelets and plasma |
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Authors: | Iftekhar Alam J Bryan Smith Melvin J Silver |
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Affiliation: | 1. Cardeza Foundation, Thomas Jefferson University, 19107, Philadelphia, PA 2. Department of Pharmacology, Thomas Jefferson University, 19107, Philadelphia, PA
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Abstract: | High performance liquid chromatography in combination with a radioactivity detector was used to study the metabolism of platelet-activating factor (1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine) by washed platelets, platelet-free plasma and platelet-rich plasma obtained from rabbits and humans. Degradation of platelet-activating factor in plasma was completely inhibited by diisopropylfluorophosphate and was partially inhibited by ethylenediamine tetraacetic acid. Washed platelets metabolized platelet-activating factor not only to the 2-lyso compound but also, by reacylation of this lyso intermediate, to an analogue of platelet-activating factor probably containing a long-chain acyl group at thesn-2 position. These transformations occurred, but to a lesser extent, in platelet-rich plasma. |
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