16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique |
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Authors: | Oto Michiei Suda Wataru Shinoyama Hirofumi |
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Affiliation: | Department of Biotechnology, Tokyo Technical College, Higashi, Kunitachi-shi, Tokyo 186-0002, Japan. ooto@tera-house.ac.jp |
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Abstract: | We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community. |
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