| 正交实验设计优化穿心莲总黄酮提取工艺 |
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| 引用本文： | 黄琼,田玉红,刘玉梅. 正交实验设计优化穿心莲总黄酮提取工艺[J]. 广州化工, 2011, 39(11): 71-72 |
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| 作者姓名： | 黄琼 田玉红 刘玉梅 |
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| 作者单位： | 广西工学院生物与化学工程系,广西柳州,545006 |
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| 基金项目： | 广西工学院博士基金项目 |
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| 摘 要： | 探讨了用乙醇回流提取穿心莲黄酮类化合物，找出最佳提取条件。通过正交实验探索乙醇回流法提取穿心莲黄酮类化合物的最佳工艺条件，采用紫外分光光度法测定总黄酮含量。影响实验因素的主次顺序是：固液比〉浸提时间〉乙醇浓度〉浸提温度，其中固液比对实验指标有显著性的影响。最佳提取工艺为固液比1：20、浸提时间4h、乙醇浓度60％、浸提温度50℃。
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| 关 键 词： | 穿心莲 总黄酮 提取工艺 正交试验 |
Optimization Extracting Process of Total Flavones from Andrographis Paniculata by Orthogonal Design |
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| HUANG Qiong,TIAN Yu-hong,LIU Yu-mei. Optimization Extracting Process of Total Flavones from Andrographis Paniculata by Orthogonal Design[J]. GuangZhou Chemical Industry and Technology, 2011, 39(11): 71-72 |
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| Authors: | HUANG Qiong TIAN Yu-hong LIU Yu-mei |
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| Affiliation: | (Deptartment of Biological and Chemical Engineering,Guangxi University of Technology,Guangxi Liuzhou 545006,China) |
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| Abstract: | Extracting process of total flavones from Andrographis paniculata was studied.Through orthogonal test to determine Andrographis paniculata of total flavonoids in the best extracting process,rating content of Andrographis paniculata was determined by ultraviolet spectrophotometric method.The effects of all factors on extract rate of total flavones from bid to small in order was solid-liquid ratio>time>ethanol concentration>temperature.The solid-liquid ratio of extraction affected the total flavones significantly.The optimized extraction process was ethanol of 60%,temperature of 50 ℃,time of 4 h,and solid-liquid ratio of 1:20. |
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| Keywords: | Andrographis paniculata flavones extraction process orthogonal design |
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