黔江烟田病毒与周边作物及杂草携毒关系分析

为明确烟田烟叶携带病毒与周边作物和杂草携带病毒的亲缘关系，利用RT-PCR克隆烟田烟叶与周边杂草和作物携带病毒外壳蛋白（CP），通过MEGA序列比对分析黔江烟田烟叶和周边杂草与作物所带病毒种类及其亲缘关系。结果显示，黔江地区烟草感染病毒有烟草普通花叶病毒（TMV）、黄瓜花叶病毒（CMV）和马铃薯Y病毒（PVY）；在烟田周边杂草和作物番薯、四季豆、糯米团、三悬叶钩草、杂草中检测到了TMV；自番薯、四季豆、蓬蘽、千里光、糯米团、三悬叶钩草中扩增出CMV；自番薯、四季豆、假酸浆、杂草中扩增出了PVY。进化关系分析显示，三悬叶钩草、四季豆、糯米团以及番薯为黔江烟田烟叶TMV的主要来源，蓬蘽为烟叶病毒病CMV的侵染源，假酸浆为烟田PVY的主要来源。

Analysis of Virus Carrying Relationship between Tobacco and Neighboring Crops and Weeds in Tobacco Fields of Qianjiang,Chongqing

To clarifying the genetic relationship between the viruses in tobacco leaves and the viruses in neighboring crops and weeds, RT-PCR was used to detect and clone virus CPs in tobacco leaves and neighboring crops and weeds. MEGA was employed to analyze the relationships between viruses from tobacco leaves and neighboring crops and weeds in Qianjiang. The results showed that tobacco leaves in Qianjiang area contained TMV, CMV and PVY. In addition, TMV CP was isolated and cloned from Gonostegia hirta (Bl.) Miq., Rubus corchorifolius, Ipomoea batatas Lam., and Phaseolus vulgaris. CMV CP was isolated and cloned from Rubus hirsutus Thunb, Senecio scandens Buch.-Ham. ex D. Don, Gonostegia hirta (Bl.) Miq., Rubus corchorifolius, Ipomoea batatas Lam. and Phaseolus vulgaris, and PVY CP was isolated and cloned from Nicandra physalodes (Linn.) Gaertn., Ipomoea batatas Lam, weeds and Phaseolus vulgaris. Through the analysis of the evolutionary relationship, it can be concluded that Rubus corchorifolius, Phaseolus vulgaris, Gonostegia hirta (Bl.) Miq. and Ipomoea batatas Lam. are the main sources of TMV in tobacco fields, Rubus hirsutus Thunb is one of the sources of infection of CMV in tobacco fields, and Nicandra physalodes (Linn.) Gaertn. is one of the main sources of PVY in tobacco fields in Qianjiang.