Characteristics of Enterotoxigenic Coagulase Positive Staphylococci Isolated from Bovine Milk in Cases of Subclinical Mastitis |
| |
| Affiliation: | 1. Ekolab Ltd, Industrijsko naselje bb, 11213 Padinska skela, Serbia;2. University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobodjenja 18, 11000 Belgrade, Serbia;3. Institute of Meat Hygiene and Technology, Kacanskog 13, 11040 Belgrade, Serbia;4. City Institute of Public Health, Bulevar Despota Stefana 61, 11200 Belgrade, Serbia |
| |
| Abstract: | Coagulase positive staphylococci are the most common cause of bovine subclinical mastitis. The goal of this study was to investigate if isolates from milk in cases of mastitis synthesize enterotoxins and express resistance to methicillin. A total of 50 strains of coagulase positive staphylococci were tested using immunoassay ELFA technique. Ten isolates were enterotoxin positive and RealTime PCR was used to identify genes encoding enterotoxins and methicillin resistance. One isolate carried the enterotoxin B encoding gene, but none were positive for the methicillin resistance gene. All isolates were confirmed as Staphylococcus aureus biochemically and by PCR. |
| |
| Keywords: | Staphylococcus aureus mastitis enterotoxins |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
