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| 集胞藻6803在封闭式光生物反应器中的混合营养培养 | |
| 引用本文： | 王永红,李元广,施定基,沈国敏,茹炳根,张嗣良.集胞藻6803在封闭式光生物反应器中的混合营养培养[J].高技术通讯,2001,11(5):28-30. |
| 作者姓名： | 王永红 李元广 施定基 沈国敏 茹炳根 张嗣良 |
| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室上海 200237 2. 中国科学院植物研究所光合作用研究中心北京 100093 3. 北京大学生命科学学院北京 100871 |
| 基金项目： | 863计划 ( 863 819 Q 0 1)，国家自然科学基金 ( 2 98760 10 )资助项目 |
| 摘 要： | 采用摇瓶和封闭式光生物反应器进行了蓝藻基因工程常用宿主系统集胞藻Syne-chocystis 6803的混合营养培养研究，在摇瓶中对集胞藻6803利用有机碳源特性进行研究的结果表明，光照是集胞藻6803利用葡萄糖的必需条件，混合营养培养是集胞藻6803的最佳培养方式，以葡萄糖为基质在2.5L封闭式光生物反应器中经过58.5h的混合营养培养，集胞藻6803的藻细胞密度和平均生长速度分别达到2.50g/L和1.01g/L/d，分别是光自养培养同期的9倍和11倍，这表明封闭式光生物反应器混合营养培养方式在促进集胞藻6803生长上有显著作用。 |
| 关 键 词： | 集胞藻6803 混合营养培养 光生物反应器 葡萄糖 蓝藻 |
Mixotrophic Cultivation of Synechocystis sp. PCC 6803 in an Enclosed Photobioreactor |
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| Wang Yonghong,Li Yuanguang,Shi Dingji,Shen Guomin,Ru Binggen ,Zhang Siliang.Mixotrophic Cultivation of Synechocystis sp. PCC 6803 in an Enclosed Photobioreactor[J].High Technology Letters,2001,11(5):28-30. | |
| Authors: | Wang Yonghong Li Yuanguang Shi Dingji Shen Guomin Ru Binggen Zhang Siliang |
| Affiliation: | Wang Yonghong,Li Yuanguang,Shi Dingji,Shen Guomin,Ru Binggen **,Zhang Siliang |
| Abstract: | Studies on the mixortophic cultivation of Synechocystis sp. PCC6803 in shake flasks and a 2.5L enclosed photobioreactor were carried out. The results from mixotrophic cultivations in shake flasks showed that light was necessary for mixotrophic growth of Synechocystis sp. PCC6803; compared with sucrose, glucose was a better kind of substance for Synechocystis sp. PCC6803 mixotrophically growing. In 2.5L enclosed photobioreactor, after 58.5h mixotrophic cultivation, 2.50g/L cell density and 1.01g/L/d were achieved, which were respectively 9 times and 11 times as much as those in photoautotrophic cultivation. On the basis of above results, it was concluded that mixotrophic cultivation system had an advantage over photoautotrophic cultivation system. |
| Keywords: | Synechocystis sp PCC6803 Mixotrophic Photobioreactor Glucose |
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