| 多种致病菌同步检测方法的研究进展 |
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| 引用本文： | 孙秀兰,张芳,张银志.多种致病菌同步检测方法的研究进展[J].食品与生物技术学报,2012,31(5):449-454. |
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| 作者姓名： | 孙秀兰 张芳 张银志 |
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| 作者单位： | 1. 食品科学与技术国家重点实验室,江南大学,江苏无锡214122;江南大学食品学院,江苏无锡214122
2. 食品科学与技术国家重点实验室,江南大学,江苏无锡214122 |
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| 基金项目： | 国家自然科学基金，公益性行业(农业)科研专项项目 |
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| 摘 要： | 概述了致病菌检测的对象,包括细菌菌群的形态及细菌本身、细菌的DNA、细菌的代谢物;同时介绍了可同步检测多种致病菌的方法,主要有电化学DNA传感器、适配体电化学传感器、免疫传感器检测技术、多重PCR检测、表面增强拉曼光谱检测;最后总结检测方法未来的发展方向。
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| 关 键 词： | 多种致病菌 同步检测 |
Research Advance Study of the Synchronous Detection of Multiplex Foodborne Pathogens |
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| SUN Xiu-lan,ZHANG Fang and ZHANG Yin-zhi.Research Advance Study of the Synchronous Detection of Multiplex Foodborne Pathogens[J].Journal of Food Science and Biotechnology,2012,31(5):449-454. |
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| Authors: | SUN Xiu-lan ZHANG Fang and ZHANG Yin-zhi |
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| Affiliation: | 1(1.State Key Laboratory of Food Science and Technology,Jiang Nan University,Wu Xi 214063,China;2.School of Food Science and Technology,Jiangnan University,Wuxi 214036,China) |
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| Abstract: | In this review,the detection targets such as bacteria colonies,bacteria cell i,DNA and bacteria metabolic products are firstly introduced.Then thedetection methods which can synchronously indentify Multiplex Food-borne Pathogens electrochemical DNA biosensor,Aptamer biosensor,Electrochemical immunosensor,Surface plasmon resonance biosensor and Multiplex PCR were summarized.Based on the above progress,the the challenge and perspective of detection method were put forward. |
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| Keywords: | detection targets multiplex foodborne pathogens synchronous detection |
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