Rapid detection of aflatoxin-producing strains of the Aspergillus flavus group isolated from mixed feed and cereal grain in Spain

A total of 133 samples (mixed feeds and cereal grains) were examined in order to detect the incidence of strains of the Aspergillus flavus group. The ability to produce aflatoxin was tested in all strains isolated on cracked rice, aflatoxin-producing-ability (APA) medium and glucose-yeast extract agar (GYA) medium. Ten out of the 67 isolations were aflatoxin-producing strains in rice and GYA medium; only three of them were aflatoxin-positive on the APA test. Of those isolated 95% were identified as A. flavus. The GYA medium is the most efficient and easiest way to detect B₁, B₂, G₁ and G₂ aflatoxin-producing-strains.