HPLC Separation of cis- and trans-Phylloquinone

This work deals with the optimization of the separation between the two isomers of phylloquinone by High Performance Liquid Chromatography regarding resolution, peak separation, retention time and peak height per quantity of sample injected. As an application of the separation method the proportions of cis- and trans-phylloquinone in a fat emulsion for parenteral nutrition, also containing the other fat soluble vitamins A, D₂ and E, were determined.