Stearoyl-coenzyme A desaturase activity in novikoff hepatoma

The activities of microsomal stearoyl-CoA desaturation, NADH-cytochrome b₅ reductase, NADH-cytochrome c reductase, and the content of cytochrome b₅ were similar in livers of normal and host rats. On the other hand, stearoyl-CoA desaturation activity was absent in Novikoff hepatoma. The activities of NADH-cytochrome b₅ and NADH-cytochrome c reductases in the hepatoma microsomes were 4.8% and 2.2%, respectively, of those in normal liver. Furthermore, in hepatoma microsomes, cytochrome b₅ was absent. An active stearoyl-CoA desaturation was reconstituted only on addition of both cytochrome b₅ and the terminal desaturase enzyme to the hepatoma microsomes. These results indicated that a complete absence of cytochrome b₅ and terminal desaturase is responsible for the lack of stearoyl-CoA desaturation in Novikoff hepatoma microsomes.