Preparation of fluorescence starch-nanoparticle and its application as plant transgenic vehicle

Starch-nanoparticles were synthesized in water-in-oil microemusion at room temperature,and the starch-nanoparticles were coated with poly-L-lysine.The surface of the starch-nanoparticles was combined with fluorescence material Ru(bpy)32+·6H2O,and then the particles were characterized via transmission electyron microscope.The fluorescence nanoparticles were conjugasted with plasmid DNA to form complexes,and then treated with ultrasound and Dnase I.pEGAD plasmid DNA-nanoparticle complexes wereco-cultured with plant suspension cells of Dioscrea Zigiberensis G H Wright,and treated with ultrasound.The results show that the diameter of the fluorescence starch-nanoparticles in 50-100nm.DNA-nanoparticle complexes can protect DNA from ultrasound damage as well as from Dnase I cleavage.Mediated by ultrasound,pEGAD plasmid DNA-nanoparticle complexes can pierce into the cell wall,cell membrane and nucleus membrane of plant suspension cells.Thegreen fluorescence protein(GFP)gene at a high frequency exceeds 5%.This nano-biomaterial can efficiently solve the problem that exterior genes cannot traverse the plant cell wall easily.