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CARBON SOURCES AND THEIR EFFECT ON GROWTH, ACETIC ACID AND ETHANOL PRODUCTION BY BRETTANOMYCES BRUXELLENSIS IN BATCH CULTURE
Authors:MG AGUILAR USCANGA  BI ESCUDERO ABARCA  J GOMEZ RODRIGUEZ  R CORTES GARCIA
Affiliation:Instituto Tecnológico de Veracruz: Unidad de Investigación en Alimentos Dpto. Ing. Eléctrica y Electrónica Miguel A. de Quevedo 2779, Veracruz Ver. Cp. 91897, México; Centro Virtual de Investigación Multidisciplinaria Universidad Veracruzana Región Xalapa Veracruz, Mexico
Abstract:The influence of available low‐cost carbohydrates as carbon sources on Brettanomyces bruxellensis growth, acetic acid and ethanol production was studied in order to ascertain the viability of this yeast to eventually become an industrial acetic acid producer. Six different raw materials were included as carbon sources (glucose, sugarcane molasses, refined cane sugar, pineapple, sugarcane and beet juices). B. bruxellensis develops in a complex culture medium like plant juices and sugarcane molasses better than in a medium with a simple carbohydrate such as glucose. The maximum acid acetic yield (0.24 g/g) and productivity (0.14 g/L/h) were attained in tests carried out with sugarcane molasses containing 60 g/L sucrose. The strain produced low levels of ethanol in a refined sugarcane medium, but was able to produce a substantial quantity of acetic acid (13 g/L).
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