Radioprotection by quercetin-3-O-rutinoside,a flavonoid glycoside – A cellular and mechanistic approach |
| |
Authors: | Punit Bansal Piya Paul Amit Kunwar S Jayakumar Pawan G Nayak KI Priyadarsini MK Unnikrishnan |
| |
Affiliation: | 1. Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576 104, Karnataka, India;2. Department of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576 104, Karnataka, India;3. Radiation & Photochemistry Division, Bhabha Atomic Research Center, Mumbai 400 085, India;4. Radiation Biology & Health Sciences Division, Bhabha Atomic Research Center, Mumbai 400 085, India |
| |
Abstract: | The present study was aimed to evaluate the in vivo radioprotective efficacy of quercetin-3-O-rutinoside (PMC-1), the key bioactive constituent flavonoid glycoside isolated from the whole plant of Pilea microphylla was evaluated. In vivo survival studies established the optimum effective dose of PMC-1 at 25 mg/kg/i.p. At the optimum dose, PMC-1 prevented the depletion of endogenous antioxidants in the liver of irradiated mice. In vivo protection towards gastrointestinal tract and haematopoietic system was confirmed by the restoration of radiation-induced reduction in villi height, number of crypt cells and spleen index. PMC-1 also attenuated the radiation-induced apoptosis in spleenocytes significantly. Single cell gel electrophoresis of peripheral blood leukocytes showed inhibition of radiation-induced DNA damage by PMC-1. PMC-1 pretreatment significantly reversed the changes by increasing pro-survival (ERK) and decreasing pro-apoptotic (BAX) gene expressions compared to radiation control. Thus, PMC-1 exhibits protective effects against γ-radiation and the probable mechanism of action involves maintenance of antioxidant enzymes, prophylactic action and inhibition of apoptosis. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|