Quercitrin protects against oxidative stress-induced injury in lung fibroblast cells via up-regulation of Bcl-xL |
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Authors: | Young-Min Ham Weon-Jong Yoon Soo-Yeong Park Gwan-Pil Song Yong-Hwan Jung You-Jin Jeon Sung-Myung Kang Kil-Nam Kim |
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Affiliation: | 1. Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 699-943, Republic of Korea;2. Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea |
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Abstract: | The cytoprotective effect of quercitrin (QR) against oxidative stress induced cell damage by hydrogen peroxide (H2O2) in Chinese hamster lung fibroblast (V79-4) cells was investigated. QR evidenced a scavenging effect of 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, hydroxyl radicals and on intracellular ROS, and thus prevented lipid peroxidation. As a result, QR reduced H2O2-induced cell death and apoptosis in V79-4 cells. Moreover, H2O2 induced the cleavage of caspase-3, -9, and poly-ADP-ribose polymerase (PARP) and a reduction in Bcl-xL levels, whereas pretreatment with QR significantly inhibited caspase-3, -9, and PARP cleavage and the reduction in Bcl-xL levels, and ultimately ameliorated H2O2-induced apoptosis. Taken together, these results indicate that the treatment of V79-4 cells with QR can block H2O2-induced apoptosis via the regulation of Bcl-xL. QR may be exploited as a biopreservative in food applications or as a health supplement to alleviate oxidative stress. |
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