乳酸对主要发炎和抗炎细胞因子的影响

目的:通过不同给药途径观察及评价了乳酸对小鼠血清中IL-6、IFN-γ、IL-1β、TNF-α、IL-10、IL-4水平的影响,进而研究了乳酸对免疫系统的调节作用。方法:通过灌胃、腹腔注射、尾静脉注射三种途径给小鼠乳酸,分别在给药4、3、3h后取血分离血清,采用ELISA法检测血清中IL-6、IFN-γ、IL-1β、TNF-α、IL-10、IL-4的含量。结果:实验组三种给药途径对小鼠血清中IL-6、IFN-γ、IL-1β、TNF-α、IL-10、IL-4与对照组相比都有显著性变化,给药组小鼠血清中IL-6、IFN-γ、TNF-α、IL-10、IL-4含量明显高于相应的对照组,IL-1β含量显著低于对照组。结论:三种给药途径中腹腔注射的效果最为明显,静脉注射及灌胃组次之。乳酸菌发酵产物的免疫调节作用可能是通过其代谢产物--乳酸发挥作用,乳酸通过对肠粘膜系统细胞细胞因子网络和信号传递系统的调节发挥免疫调节和生理功能。

Effects of Lactic Acid on Production of Pro- and Anti- inflammatory Cytokines

Objective: To explore and evaluate the effects of lactic acid on the production level of IL-6, IFN-γ, IL-1β, TNF-α, IL-10 and IL-4 in sera of mice and the regulative effect of lactic acid on immune system. Methods: By ELISA assay the contents of IL-6, IFN-γ, IL-1β, TNF-α, IL-10 and IL-4 were examined in sera of mice after orally administration, intraperitoneal injection, intravenous injection of lactic acid to mice. Sera was separated after lactic acid was given 4, 3 and 3h. Results: It showed that the content of IL-6, IFN-γ, TNF-α, IL-10, IL-4 were more significantly increased than the control groups after orally administration, intraperitoneal injection, intravenous injection of lactic acid to mice and the content of IL-1β was more significantly decreased than control groups. Conclusion: All of the three approaches, the effect of intraperitoneal injection is the most obvious. Lactic acid is the metabolic product of Lactobacillus. The immunomolating function of Lactobacillus zymolytic food can be exerted by lactic acid, which through the cytokine secreted by immunocyte in gut mucosal system.