Abstract: | An environmental assessment of Legionella bacteria was conducted in five office buildings in the continental United States where no legionellosis was reported. The purpose of this investigation was to (i) determine the presence of Legionella bacteria in potable and non-potable water systems, (ii) provide a baseline information for management, and (iii) evaluate the effectiveness of the remedial actions taken. Water samples were collected from all possible water sources in surveyed buildings. The samples were analyzed by both direct fluorescent antibody microscopy (DFA) and the bacterial cultural method for the presence of Legionella species. Legionella bacteria were detected in some samples collected from various water distribution systems in the buildings. Remedial action was taken to eliminate these bacteria, and case-by-case results are presented. |