Fermentative hydrogen production by the newly isolated Enterobacter asburiae SNU-1 |
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Authors: | Jong-Hwan Shin Jong Hyun Yoon Eun Kyoung Ahn Mi-Sun Kim Sang Jun Sim Tai Hyun Park |
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Affiliation: | 1. School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea;2. Biomass Research Team, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea;3. Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea |
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Abstract: | A new fermentative hydrogen-producing bacterium was isolated from a domestic landfill and identified as Enterobacter asburiae using 16S rRNA gene sequencing and DNA–DNA hybridization methods. The isolated bacterium, designated as Enterobacter asburiae SNU-1, is a new species that has never been examined as a potential hydrogen-producing bacterium. This study examined the hydrogen-producing ability of Enterobacter asburiae SNU-1. During fermentation, the hydrogen was mainly produced in the stationary phase. The hydrogen yield based on the formate consumption was 0.43 mol hydrogen/mol formate. This strain was able to produce hydrogen over a wide range of pH (4–7.5), with the optimum pH being pH 7. The level of hydrogen production was also affected by the initial glucose concentration, and the optimum value was found to be 25 g glucose/l. The maximum and overall hydrogen productivities were 398 and 174 ml/l/hr, respectively, at pH 7 with an initial glucose concentration of 25 g/l. This strain could produce hydrogen from glucose and many other carbon sources such as fructose, sucrose, and sorbitol. |
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Keywords: | Enterobacter asburiae SNU-1 Hydrogen production Fermentation Formate decomposition |
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