Preparation of14C-gossypol by incorporation of acetate-1-14C and acetate-2-14C by biosynthesis

Details for the germination of cotton seedlings and their utilization for incorporating¹⁴C-labeled acetate into gossypol are presented. Methods for the isolation and purification of the biosynthesized gossypol are given. The specific activity of the gossypol obtained from root tissue and cotyledons had mean values for 8 preparations of 428.6×10³ and 6.3×10³ dpm/mg, respectively. Corresponding incorporations were 5.42 and 0.32%. Paper no. 4190, North Carolina State University Experiment Station, Raleigh, N.C.