一株降解恩诺沙星菌株的筛选鉴定及其降解条件的优化

从长期处理含恩诺沙星猪粪的黑水虻肠道中,筛选出可降解恩诺沙星的菌株,并对菌株降解过程中的环境因素进行优化,获得较优的降解条件,为研究恩诺沙星生物降解的途径提供理论基础.将黑水虻肠液加入含有恩诺沙星的培养基中培养,经过两次筛选,从若干株具有降解恩诺沙星能力的菌种中挑选降解能力最强的一株菌株,通过单因素实验确定该菌株最适降...

Screening and Identification of Enrofloxacin Degrading Strain and Optimization of Its Degradation Conditions

In order to study the biodegradation of enrofloxacin,a strain was screened from the the gut of black soldier fly,which had been used to treat the pig manure containing enrofloxacin for a long time.The intestinal juice of black soldier fly was cultured in the medium containing enrofloxacin.After twice screening,the strain with the strongest ability to degrade enrofloxacin was selected from several strains with the ability to degrade enrofloxacin.The optimal degradation conditions were determined by single factor experiment:Temperature,initial pH,inoculum amount and shaking speed.According to the results of single factor experiment,the optimal degradation conditions were obtained by response surface analysis.Results showed that,17 strains of enrofloxacin degrading bacteria were obtained in the initial screening,and 3 strains of enrofloxacin degrading bacteria were found in the further re-screening.BSFL-1 and BSFL-3 were identified as Enterococcus faecalis and BSFL-2 as Proteus mirabilis.The strain with the highest degradation rate was BSFL-3.By response surface analysis,the optimal degradation conditions were as follows:Temperature 33.6℃,initial pH5.8,inoculation amount 4%,shaking speed 155 r/min.Under these conditions,the degradation rate of enrofloxacin was 77.83%±0.53%.The strain with optimized degradation conditions has high degradation rate,which broadens the research field of biological degradation of enrofloxacin,and provides help for the application research of using bacterial degradation rate of enrofloxacin.