| 酶法辅助提取花生壳中木犀草素的工艺研究 |
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| 引用本文： | 熊清平,张强华,石莹莹,夏飞,王倩.酶法辅助提取花生壳中木犀草素的工艺研究[J].中国酿造,2012,31(1):46-49. |
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| 作者姓名： | 熊清平 张强华 石莹莹 夏飞 王倩 |
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| 作者单位： | 淮阴工学院生化学院,江苏省凹土资源利用重点实验室,江苏淮安223003 |
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| 基金项目： | 国家科技部十二五支撑项目(2011BA101B02);江苏省淮安市工业基金项目(HAG0706) |
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| 摘 要： | 目的：研究纤维素酶法辅助提取花生壳中木犀草素的最佳工艺条件。方法：采用HPLC法测定木犀草素含量,以提取率为指标,分别考察料液比、加酶量、pH值、温度、时间对纤维素酶酶解反应的影响,并将筛选工艺与目前文献的乙醇直接提工艺进行对比分析。结果：纤维素酶酶解花生壳的最佳工艺条件：料液比为1∶8（w/v）、酶用量为25U/g药材的量、酶解pH值为4.8、酶解温度50℃、酶解时间为6h。结论：与传统乙醇直接提取工艺相比,酶法辅助提取工艺提取花生壳中木犀草素的提取率提高了2.15倍。
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| 关 键 词： | 纤维素酶 提取 花生壳 木犀草素 |
Enzyme-assisted extraction of luteolin from peanut hull |
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| XIONG Qingping
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ZHANG Qianghua
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SHI Yingying
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XIA Fei
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WANG Qian.Enzyme-assisted extraction of luteolin from peanut hull[J].China Brewing,2012,31(1):46-49. |
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| Authors: | XIONG Qingping ZHANG Qianghua SHI Yingying XIA Fei WANG Qian |
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| Affiliation: | (Huaiyin Institute of Technology,Key Laboratory for Attapulgite and Applied Technology of Jiangsu Prowince,Huai’an 223003,China) |
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| Abstract: | Objective: The optimum conditions for cellulase-assisted extraction of luteolin from peanut hull were studied.Methods: The luteolin content was determined by HPLC.According to extraction rate,the effects of solid material-liquid ratio,enzyme concentration,pH value,temperature and reaction time on cellulase reaction were studied.Besides,the enzyme-assisted extraction was analyzed with the comparison of ethanol extraction technology.Results: The optimum reaction conditions of cellulase were determined as follows: solid material-liquid ratio 1:8(w/v),25U cellulase added for 1g material,pH value 4.8,temperature 50℃ and reaction time 6h.Conclusion: Compared with the traditional ethanol extraction,the luteolin yield was improved by 2.15times. |
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| Keywords: | cellulase extraction peanut hull luteolin |
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