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MRS培养基组分对Lactobacillus J23合成抗菌肽Bac-J23的影响
引用本文:易华西,韩雪,杜明,张兰威.MRS培养基组分对Lactobacillus J23合成抗菌肽Bac-J23的影响[J].中国酿造,2012,31(5):56-58.
作者姓名:易华西  韩雪  杜明  张兰威
作者单位:哈尔滨工业大学食品科学与工程学院中法乳国际联合实验室,黑龙江哈尔滨,150090
基金项目:国家自然基金项目(30900996);中央高校基本科研业务费专项资金项目(HIT.NSRIF.2011 85)
摘    要:研究了培养基MRS组分对乳酸菌Lactobacillus J23产生广谱抗菌肽Bac-J23的影响,并对其组成进行了优化.结果表明,当培养基中浓度为5g/L的乙酸钠对Bac-J23的合成具有刺激诱导作用,蔗糖对Bac-J23合成的最有利,酵母提取物对Lactobacillus J23的生长和Bac-J23的合成均具有明显的促进作用,KH2PO4(15g/L~20g/L)有利于Bac-J23的合成,MgSO4 (0.2g/L)对合成Bac-J23最有利,MnSO4和Tween-80对Bac-J23合成及Lactobacillus J23生长均没有明显影响.

关 键 词:MRS培养基  Lactobacillus  J23  抗菌肽

Effect of MRS medium components on bacteriocin Bac-J23 production from Lactobacillus J23
YI huaxi , HAN Xue , DU Ming , ZHANG Lanwei.Effect of MRS medium components on bacteriocin Bac-J23 production from Lactobacillus J23[J].China Brewing,2012,31(5):56-58.
Authors:YI huaxi  HAN Xue  DU Ming  ZHANG Lanwei
Affiliation:(School of Food Science & Engineering of Harbin Institute of Technology,Harbin 150090,China)
Abstract:The effect of MRS medium ingredient on the bacteriocin Bac-J23 produced by Lactobacillus J23 were studied.The results showed that 5g/L acetic acid sodium,sucrose and Yeast extraction could stimulate the growth of strain Lactobacillus J23 and induce Bac-J23 production.Higher concentration KH2PO4(15g/L~20g/L)and 0.2g/L MgSO4 were propitious to Bac-J23 production,while MnSO4 and Tween-80 had no influence to the growth of Lactobacillus J23 and the production of Bac-J23.
Keywords:MRS medium  Lactobacillus J23  bacteriocin
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