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Optimized conditions for the supplementation of human-induced pluripotent stem cell cultures with a GSK-3 inhibitor during embryoid body formation with the aim of inducing differentiation into mesodermal and cardiac lineage
Affiliation:1. Faculty of Life and Environmental Sciences, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-4-37 Takeda, Kofu, Yamanashi 400-8510, Japan;2. Advanced Biotechnology Center, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan;1. Laboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China;2. Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia;1. Department of Biological Resource Sciences, Akita Prefectural University, Nakano Shimoshinjyo, Akita 010-0195, Japan;2. Akita Research Institute for Food & Brewing, 4-26 Sanuki, Araya-machi, Akita 010-1623, Japan;3. National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima 739-0046, Japan;1. Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-naka, Kita-ku, Okayama 700-8530, Japan;2. Laboratory of Manufacturing Engineering, ENIT-University of Toulouse III, 47 av. d''Azereix, BP 1629-65016, Tarbes CEDEX, France;1. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan;2. HAKUTSURU SAKE Brewing Co., Ltd., 4-5-5 Sumiyoshi Minamimachi, Higashinada-ku, Kobe, Hyogo 658-0041, Japan;3. Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki Aza Aoba, Aoba-ku, Sendai, Miyagi 980-0845, Japan;4. Osaka University Shimadzu Analytical Innovation Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan;1. Department of Materials Science and Technology, Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan;2. Graduate School of Technology, Niigata Institute of Technology, Niigata 945-1195, Japan
Abstract:
Keywords:GSK3 inhibitor  CHIR99021  Mesoderm  Cardiomyocyte  Embryoid body
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