透明质酸发酵过程控制的研究

进行了兽疫链球菌NW-162高密度发酵HA的工艺研究,通过发酵过程中不同饱和度的溶解氧和不同pH调控方法对该菌株发酵生产透明质酸的影响进行了研究。实验表明,采用30%饱和度的溶解氧和在发酵过程的0~30 h,调pH 7.2,发酵后期调pH 6.8的调控方法,OD660可达0.84,透明质酸产量为0.532 g/L。在此条件下,有利于细胞生长和产物表达,降低乙酸等有害代谢副产物积累。

Research on control of fermentation of NW-162 expressing HA

The high cell-density fermentation process for producing hyaluronic acid with a streptococcus zooepidemius mutant NW-162 was studied.The different dissolved oxygen(DO) and different pH was optimized during fermentation process.The result showed the maximal yield of hyaluronic acid was 0.532 g/L and DO660 value reached 0.84,when the dissolved oxygen was controlled at 30% and pH value was at pre-term and mid-term pH 7.2,final-term pH 6.8 during different terms of fermentation.Under the conditions,it was beneficial to the growth of cell and aim product expression and it could reduce the accumulation of acetic acid.