| 氯霉素单克隆抗体制备以及化学发光检测试剂盒的研制 |
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| 引用本文： | 吴小胜,焦强,万宇平,王兆芹,王琳琛,丛倩千,朱亮亮,何方洋. 氯霉素单克隆抗体制备以及化学发光检测试剂盒的研制[J]. 中国酿造, 2019, 0(4): 179-182 |
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| 作者姓名： | 吴小胜 焦强 万宇平 王兆芹 王琳琛 丛倩千 朱亮亮 何方洋 |
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| 作者单位： | 北京勤邦生物技术有限公司;北京市食品安全免疫快速检测工程技术研究中心;河南省口岸食品检验检测所 |
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| 基金项目： | 科技北京百名领军人才培养工程(Z171100001117158)项目资助 |
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| 摘 要： | 通过用钯炭催化氯霉素,通入氢气,制备出氯霉素半抗原,通过动物免疫、细胞融合,获得杂交瘤细胞株,再由杂交瘤细胞株分泌得到抗氯霉素单克隆抗体,从而制备出能够检测鱼肉和虾肉样本中氯霉素药物的化学发光试剂盒。试剂盒对鱼肉的检测限为0.047μg/kg,对虾肉的检测限为0.045μg/kg,对鱼肉的回收率为83.5%～109.3%,批内、批间相对标准偏差(RSD)范围均<10%。氯霉素单克隆抗体与棕榈氯霉素、甲砜霉素、氟甲砜霉素、氟苯尼考胺的交叉反应率均<1%。试剂盒能够在4℃保存1年。
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| 关 键 词： | 氯霉素 单克隆抗体 化学发光检测试剂盒 |
Preparation of chloramphenicol monoclonal antibodies and chemiluminescence immune assay kit |
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| WU Xiaosheng,JIAO Qiang,WAN Yuping,WANG Zhaoqin,WANG Linchen,CONG Qianqian,ZHU Liangliang,HE Fangyang. Preparation of chloramphenicol monoclonal antibodies and chemiluminescence immune assay kit[J]. China Brewing, 2019, 0(4): 179-182 |
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| Authors: | WU Xiaosheng JIAO Qiang WAN Yuping WANG Zhaoqin WANG Linchen CONG Qianqian ZHU Liangliang HE Fangyang |
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| Affiliation: | (Beijing Kwinbon Biotechnology Company,Beijing 102206,China;Food Inspection and Testing institute of Henan Province,Zhengzhou 450003,China;Beijing Engineering Research Centre of Food Safety Immunodetection,Beijing 102206,China) |
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| Abstract: | WU Xiaosheng;JIAO Qiang;WAN Yuping;WANG Zhaoqin;WANG Linchen;CONG Qianqian;ZHU Liangliang;HE Fangyang(Beijing Kwinbon Biotechnology Company,Beijing 102206,China;Food Inspection and Testing institute of Henan Province,Zhengzhou 450003,China;Beijing Engineering Research Centre of Food Safety Immunodetection,Beijing 102206,China) |
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| Keywords: | chloramphenicol monoclonal antibodies chemiluminescence immune assay kit |
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